Improve the utility of multiparameter flow cytometry (MFC) for clinical diagnosis
Our clinical diagnostic laboratory routinely uses multiparameter flow cytometry (MFC) to detect pathology of bone marrow samples for hematopoietic diseases diagnosis and selection of the optimal therapy.
The wide clinical and biological heterogeneity of bone marrow samples make MFC difficult to standardise, with results often depending on the experience of the analyst.
We proposed (based on the evaluation of the lineage specific markers by MFC) to develop maturation databases for myeloid, lymphoid and erythroid lineages with bone marrow from "healthy" donors (without any evidence of a hematopoietic disease).
Patient bone marrow will be analysed in the same manner and compared against the maturation databases.
Quantitative phenotypic abnormalities can be identified and those deviating significantly compared with data of normal bone marrow samples should be considered indicative of pathology.
A robust method of analysis for each lineage within bone marrow should be applicable for routine diagnostic use. The reduction of investigator subjectivity in data analysis is an important advantage of this approach.
